In Vitro Methods in Aquatic Toxicology

Carmel Mothersill and Brian Austin

In this ground-breaking work, focus is placed on in vitro approaches to the study of the toxicology of polluting agents in the aquatic environment. Methods are critically discussed and both the shortcomings and advantages are presented. The reader is asked to consider the appropriateness of a method in relation to the question being asked, and shown how to develop relevant in vitro models. The importance of validating and interpreting in vitro data is stressed.

As well as providing this valuable assessment of methods and data, In Vitro Methods in Aquatic Toxicology also presents a new philosophical approach central to ecotoxicology, embedding in vitro exotoxicogical methods firmly in the context of an integrated approach to determining the impacts of toxins in the environment.

Table of contents:

• Contents
• Preface
• List of contributors
• List of abbreviations
• List of figures
• List of tables
• List of colour plates
  1. Introduction: basic requirements for in vitro applications in aquatic eco-toxicology (C. Mothersill and B. Austin)
  PART I: CULTURE TECHNIQUES
  2. Basic methods and media for eukaryotic cells in vitro (B. Austin and C. Mothersill)
  3. Development of primary tissue culture techniques for use in ecotoxicology: some considerations necessary prior to model selection (C. Mothersill and C. Seymour)
  PART II: END POINT AND TOOLS FOR IN VITRO TOXICOLOGY
  5. Cultured epithelia from fish gills (P. Part and C.M. Wood)
  6. Applications of in vitro techniques in studies of biomarkers and eco-toxicology (D. Sheehan)
  7. End points for in vitro toxicity testing with fish cells (H. Segner and T.Braunbeck)
  8. Toxicogenomic technologies for in vitro aquatic toxicology (L.E.J. Lee, M.M. Vijayan and B. Dixon)
  PART III: SPECIFIC TOXINS AND TOXIC GROUPS
  9. Delayed and indirect genotoxicity: a paradigm shift in toxicology? (E.G. Wright)
  10. Toxicogenomic applications in environmental toxicology (L.N. Moens, K. Van der Ven, M. Caturla and W.M. De Coen)
  11. Biomarkers to assess endocrine disruption of reproduction in bivalves (F. Gagne and C. Blaise)
  12. Fish cells used to detect aquatic carcinogens and genotoxic agents (A. Castano, C. Becerril and M.T. Llorente)
  13. In vitro biomarkers of radiation exposure in the environment (B.A. Ulsh and D.R. Boreham)
  14. Ecotoxicological characterization of complex mixtures (G. Repetto, A. del Peso and A. Jos)
  PART IV: PRACTICAL ISSUES
  15. In vitro ecotoxicological assessment of aquatic sediments (S. Ni Shuilleabhain, M. Davoren, M.G.J. Hartl and J. O'Halloran)
  16. In vitro/in vivo bridging approaches - validating the relevance of in vitro techniques with references to the whole organism in the natural environment (M. Kilemade and B. Quinn)
  17. Cell culture approaches in aquatic immunotoxicology (N.C. Bols, S.J. DeWitte-Orr, J.L. Brubacher, B. Dixon and R.C. Ganassin)
  18. Validation of alternative methods to animal testing (M. Halder and A. Worth)
  19. Legal and ethical aspects of environmental toxicology with specific references to in vitro approaches (C. Seymour)
  20. The mathematics of cell growth in culture (R. Ocone, A. Kummer and X. Yang)
  21. Conclusions (B. Austin, C. Seymour and C. Mothersill)
• Index

Extent: 512 pages
Binding: hardback
Publication Date: August 2003
ISBN: 978-3-540-02357-9

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